Renylab Cytological Dyes

Renylab’s Papanicolaou dyes manufactured with high quality raw materials, resulting in a clear and harmonic coloring.

The Papanicolaou test, also known as Oncotic Colpocytology and Cervical Cytology, aims to perform the early diagnosis of lesions or cytological changes that can cause cervical cancer, being the main strategy for the early detection of these changes. This test should be performed annually by all women, who have or have had any sexual contact.

Microscopic observation aims to check cells to identify abnormalities, morphological or nuclear changes. The examination is performed through a collection of endocervical and ectocervical cells, where the samples are placed on a slide, fixed and stained with a combination of dyes EA-36, OG-06 (Orange G) and Harris Hematoxylin. After staining, cells are inspected microscopically.

Resulting from the combination of several dyes, EA-36 exerts a polychromatic staining in the cytoplasm of cells, Eosin stains the cytoplasm of superficial cells, nucleoli, endocervical mucin and cilia, giving a pinkish tone to these components, while the light green acts on parabasal and intermediate squamous cells, columnar cells and histiocytes giving a bluish green hue.

Orange G (OG-06) is an acid monochromatic dye capable of staining red blood cells and keratinized cells giving these cells an orange tint.

Harris’ hematoxylin has an affinity for acidic cellular components, which gives it a violet color. Hematoxylin intensely colors cell nuclei, helping to visualize nuclear changes.

Cytopathology analyzes individualized cells, peeled, expelled or removed from the surface of organs from different parts of the body. As biological materials have different characteristics, due to different forms of organization and composition, the collection of material for cytological analysis is a fundamental step in this process. There are specific methods for collecting different materials. In addition, at this stage, the types of procedures most suitable for the analysis of cytological preparations are defined.

The Papanicolaou method covers five steps:

• Hydration: this step requires the gradual replenishment of the water in the cells by means of alcoholic baths of decreasing concentrations to the distilled water.
• Nuclear staining: hydrated cells can now receive an aqueous dye to stain the nuclei (Harris hematoxylin).
• Dehydration: to receive cytoplasmic alcoholic dyes, we must now remove the water from the cells with alcoholic baths of increasing concentrations.
• Cytoplasmic staining: in this step, the cytoplasm of the cells is stained by the dyes Orange G and EA-65, in order to differentiate the cytoplasm of the cells according to their maturity and metabolism with different shades.
• Dehydration, clarification and sealing: water must now be removed with increasing alcohol concentrations, clarified and sealed with permanent hydrophobic media.

The quality of cytological staining is directly related to the dye characteristics of the dyes, the sample processing (thickness of the smears) and the fixation. These precautions must be observed to avoid artifacts and difficulty in analyzing the material.

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Address: Renylab Química e Farmacêutica- BR-040, Km 697 – Caiçaras, Barbacena – MG, 36205-666